Knockdown of HSPA1A promotes bortezomib-induced growth inhibition <i>in vivo</i>.

<p><b>A.</b> Effects of bortezomib on HSPA1A induction. Animals were injected twice with 1 mg/kg bortezomib (3 days apart), tumor RNA was harvested, and HSPA1A expression was measured by quantitative RT-PCR. Mean ± SEM, n = 3. RQ = relative quantity (to GAPDH). <b>B.</b> Effects of bortezomib on tumor growth. Athymic nude mice were inoculated s.c. with 253JB-V.KD9 or 253JB-V.NT cancer cells. When tumors became palpable (5–7 days), the mice were treated i.v. biweekly with bortezomib at 1 mg/kg/dose or with saline control. Tumor volumes were measured twice a week after the start of treatment. Values represent mean ± SE (N = 5).