Perforin is required for CD8+ T cell induced pathology in <i>L. </i>braziliensis infection.

<p><i>Rag1−/−</i> mice were infected with <i>L. braziliensis</i> in the ear, and reconstituted with either WT or <i>Prf−/−</i> CD8+ T cells. (A) Ear thickness at the site of infection. (B) Representative images of leishmanial lesions at 5 weeks post infection. At 7 weeks post infection mice were euthanized and shown are: (C) parasite burden in the lesions; representative (D) contour plots and (E) bar graph of CD8+ T cell in the lesion (pregated on: Live/Singlets/CD45+/CD8β+ cells). Graphs are representative data from one of three independent experiments (n = 5). <i>Rag1−/−</i> mice were infected with <i>L. braziliensis</i> in the ear, and reconstituted with either WT, IFN-γ−/−, or IL-17−/− CD8+ T cells. (F,H) Ear thickness at the site of infection; (G,I) Parasite burden in the lesions at 7 weeks post infection. Data from one (F,G) experiment or one representative experiment of two (H,I) (n = 5) are presented. <i>*p<0.05.</i>