Detection of oxidative DNA base lesions at telomeres in mice.

<p>Genomic DNA is detected for Endonuclease III-sensitive DNA lesions per kilobase of telomeric DNA by a modified quantitative telomere PCR method. (A) Endonuclease III-sensitive DNA lesions in wild-type and <i>Nth1^−/−</i> mouse kidney (n = 6 mice). (B) Endonuclease III-sensitive DNA lesions in primary MEFs (untreated) or MEFs exposed to 5 µM Benzo(a)pyrene for 24 hours followed by recovery for 0 hour or 8 hours. Each sample is analyzed in triplicate. Error bars denote standard deviation. P-values are calculated using a Student's <i>t</i>-test and adjusted using Benjamini-Hochberg False Discovery Rate-controlling method <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003639#pgen.1003639-Benjamini1" target="_blank">[57]</a>. P-values<0.05 are statistically significant using the above method.