Analysis of oncomiRs and exosomal miRNA subsets.

<p>Oncomirs belonging to the miR-17-92 cluster were analyzed for expression. (A) Box plot showing the distribution of expression scores for ∼150 miRNAs associated with cancer for KS biopsies, exosomes in KS patients, exosomes in normal human plasma and RNase-treated, exosome-depleted supernatant from control plasma. Individual circles represent individual miRNAs and their respective expression levels in the samples. Blue circles represent members of the oncogenic miR-17-92 cluster while other oncogenic miRNAs are denoted by red circles. Box plots of relative expression levels of the miR-17-92 and 106b-25 clusters in control and tumor samples are shown in human (B) and mouse (C) exosome samples. (D) A subset of miRNAs showed exclusive expression in mouse exosomes and not in plasma exosomal supernatants (free miRNAs). Expression levels in transgenic and xenograft mouse exosomes are also higher than control exosomes for these miRNAs. Sample abbreviations: exo, KS exosomes; neg, control exosomes; tumor, tumor biopsies; mock, control human or mouse plasma/serum, plasma, KS patient plasma; pleural, pleural fluid; tg, KSHV latency locus transgenic mice; tum, xenograft tumor mice; cEXO, control exosomes; cSN, control supernatant; tExo, tumor exosomes; tSN, tumor supernatant fraction.