Antiviral activity of the panel of modified tetherin proteins.

<p>(<b>A</b>) Western blot analyses of the 293T cell lysates that were cotransfected with a Vpu-deficient (HIV-1 ΔVpu) proviral pNL4-3 plasmid along with varying amounts of the indicated modified tetherin proteins. All samples were probed with an anti-HA antibody. (<b>B</b>) 293T cells were cotransfected with WT (HIV-1 WT) or Vpu-deficient (HIV-1 ΔVpu) proviral pNL4-3 plasmids along with varying amounts of the indicated modified tetherin proteins. Infectious virion yield was measured by inoculating HeLa-TZM indicator cells with culture supernatant and is given as the logarithm to the base 10 of the relative light units (RLU). (<b>C</b>) Western blot analyses of transfected 293T cell lysates and virions corresponding to the above panel. All samples were probed with an anti-CA antibody. The numbers at the bottom represent measurement of CA protein levels in virion pellets (LI-COR).