Association between changes in miRNA 3′ nucleotide additions and changes in miRNA abundance.

<p>MicroRNA 3′ additions and total abundance levels were assessed with NanoString profiling following the suppression of nucleotidyl transferases in HCT-116 cells. (A) Bars depict miRNAs with significant changes in additions compared to negative control cells with a false discovery rate <10%. The bars display the fold change in a given miRNA isomiR, with the color of the bar indicating the 3′ addition affected. The enzyme names above the bars indicate the suppressed nucleotidyl transferase. Data for miRNAs showing decreased addition are from our previous report <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0069630#pone.0069630-Wyman1" target="_blank">[25]</a>. (<b>B</b>) Boxplots comparing the abundance changes of miRNAs showing increases, decreases, or no change in 3′ additions. MicroRNAs with significant increases in 3′ additions feature a statistically significant decrease in abundance compared to miRNAs with unchanged or decreased additions (t-test, p-value <0.005). (<b>C</b>) Increased additions are associated with decreased miRNA abundance following suppression of three different nucleotidyl transferase enzymes. Shading of a box indicates a t-test p-value <0.05 when comparing the abundance in miRNAs with unchanged versus increased or decreased 3′ addition. (<b>D</b>) MicroRNAs with increased 3′ U additions show significant decreases in abundance compared to miRNAs with unchanged additions (t-test, p-value <2×10⁻⁵).