NMIIA and MLC are involved in EHEC-L-induced Stx MPC.

<p>(A) Representative IB and quantitative data show that EHEC-L-induced MPC is accompanied by a significant increase in the relative amount of NMIIA and increase in pMLC (* - significant compared to the control (p < 0.01); n ≥ 4 monolayers per each experimental condition from 3 independent experiments. (B) Representative XY confocal optical sections through the apical region of T84 cells and corresponding XZ projections show the difference in MLC (red) distribution between control and EHEC-L-treated monolayers. White arrows indicate that upon EHEC-L treatment the MLC is concentrated in an apical macropinocytic bleb. In both panels: MLC-red by AlexaFluor568; nuclei – blue by Hoechst. Analysis of 23 apical F-actin blebs in EHEC-L treated cells from 2 independent experiments show that all 23 counted apical blebs were MLC-positive.