Tai is required for <i>scrib</i>⁻+<i>ab</i> tumour overgrowth, and sufficient to cooperate with the loss of <i>scrib</i>.

<p><i>ey-FLP</i> induced eye/antennal disc clones at 5 (A,B,D,E,G) and 9 days (F) AEL, and a dorsal view of mosaic adult eyes (C). Clones are marked by GFP (white, or green in merges). Tai (A) is white (and in merges appears magenta when overlaid with GFP), and Elav (B,D,E) is white (and blue in merges – dark blue when overlaid with GFP). F-actin is red in merges in B,D–G. Brain lobes in F,G are marked by BL. GFP (panel A), Tai (panel A′), Elav (panels B,D,E), and merges (panels A″,B′,D′,E′). (A) Expression of <i>tai^RNAi</i> in clones reduces endogenous levels of Tai. (B–C) Expression of <i>tai^RNAi</i> in <i>scrib¹</i>+<i>ab</i> clones reduces clonal overgrowth (B) compared with <i>scrib¹</i>+<i>ab</i> (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003627#pgen-1003627-g001" target="_blank"><b>Figure 1G–J</b></a>), and results in the eclosion of adult flies (C). (D) Overexpression of <i>tai^FL</i> in clones does not block Elav expression, nor cause clonal overgrowth throughout an extended larval stage. (E–F) Overexpression of <i>tai^FL</i> in <i>scrib¹</i> clones promotes mutant tissue overgrowth and a block to Elav expression (E, arrowhead), eventually resulting in the formation of large tumours after an extended larval stage of development (F). (G) Wild type eye/antennal disc clones attached to the brain lobes at day 5, just before pupariation. Yellow scale bar = 50 µm.