Autoreactive CD4 T cells suppressed in treated mice.

<p>A. On day 7 after the second immunization, the PBMC and splenocytes were prepared and immunostained with anti-CD4 mAb. The samples were stained with anti-CD4-FITC mAb and analyzed by flow cytometry. Data shown are representative of 3 independent experiments. Bar, mean and SD from 3 independent experiments, each using at least three mice per group (n = 3). For statistical analysis, mice treated with B9-23/DEX were compared with that in mice treated with B9-23 group and student-t test were used between the indicated pair; *p<0.05. B. On day 7 after the second immunization, the pancreatic samples were immunostained with anti-CD4-FITC mAb and analyzed by flow cytometry. The infiltrated lymphocytes (R1) were gated for CD4 T cells analysis. The CD4 T cells (CD4⁺, R2) were counted relatively to the infiltrated lymphocytes. Data shown are representative of 3 independent experiments. Data shown are representative of 3 independent experiments. Bar, mean and SD from 3 independent experiments, each using at least three mice per group (n = 3). For statistical analysis, mice treated with B9-23/DEX were compared with that in mice treated with B9-23 group and student-t test were used between the indicated pair; **p<0.01. C. On day 7 after the second immunization, the pancreatic samples were immunostained with anti-CD8-APTC mAb and analyzed by flow cytometry. The infiltrated lymphocytes (R1) were gated for CD8 T cells analysis. The CD8 T cells (CD8⁺, R2) were counted relatively to the infiltrated lymphocytes.