Baicalein effect on astrocytic Epo/VEGF expression and its HIF1α dependency and correlation with the PHD inhibitor effect in astrocytes.

<p>(A, C) Primary cultured astrocytes were treated with baicalein (BA) at the indicated concentrations for 24 h, followed by mRNA extraction for qRT-PCR analysis of Epo, VEGF,(A) and TNFα (C) transcripts. (B) Culture medium of astrocytes was collected 24 h after the BA treatment for ELISA analysis of Epo and VEGF. (D, E) For the HIF1α dependency experiment, the scrambled RNA- or si<i>Hif1a</i>-transfected astrocytes were treated with BA (35 μM) for 24 h, followed by qRT-PCR analysis of VEGF (D) and Epo (E) mRNA. (F) Protein levels of PHD2 in neurons versus astrocytes as analyzed by Western blotting. (G) qRT-PCR of Epo and VEGF mRNA in 0.5 mM or 1.5 mM DMOG-treated neurons and astrocytes *p<0.05, **p<0.01, ***p<0.001 versus control; ^# p<0.05 and ^{# #} p<0.01 versus the Scr-BA-treated group in (D), and versus Neurons-Ctrl or Neurons-DMOG group in <b>G</b> (n=3).