Aberrant regulation of actin cytoskeleton components.

<p><b>A.</b> SQRT-PCR shows increased <i>WIPF1</i> mRNA expression in KEB-7 and EBDM-1 cell lines compared to NEB-1 wild-type keratinocytes (n = 3). <b>B.</b> SQRT-PCR shows increased <i>ARHGEF9</i> mRNA expression in KEB-7 and EBDM-1 cell lines compared to NEB-1 wild-type keratinocytes (n = 5). <b>C.</b> Immunoprecipitation and western blot show increased amounts of active (GTP-bound) Cdc42 protein in KEB-7 and EBDM-1. Annexin-I was used as a loading control. <b>D.</b> Western blot of whole-cell lysates shows increased amounts of phospho-ERM proteins in KEB-7 and EBDM-1. Annexin-I was used as a loading control. Student’s <i>t</i>-test was performed with p values: * ≤0.05, ** ≤0.01, ΔΔ ≤0.0005.