Type I and type II cytokeratin expression.

<p><b>A.</b> SQRT-PCR shows an increase in mRNA expression of type I cytokeratins <i>K14</i>, <i>15</i>, <i>16</i> and <i>17</i> in KEB-7 (K14 R125P) and EBDM-1 (K14 R125H) cell lines compared to NEB-1 wild-type keratinocytes (n = 4 to n = 7). <b>B.</b> SQRT-PCR of type II cytokeratins shows only a significant increase of <i>K5</i> mRNA expression in the KEB-7 cell line (n = 3 to n = 5). <b>C.</b> Western blot analysis from whole-cell lysates revealed increased protein expression of K14, 15 and 16 in both EBS-DM cell lines. Annexin-I was used as a loading control. Student’s <i>t</i>-test was performed with p values: * ≤0.05, ** ≤0.01, Δ≤0.001, ΔΔ≤0.0005, ΔΔΔ≤0.0001, ‡ = no significant difference between investigated cell lines.