Expression and regulation of cell-surface markers.

<p>(a) Schematic diagram showing the stages of the progression of neural stem cells and their relation to the cell surface molecular map used in flow cytometric analysis. (<b>b–c</b>) Sample flow cytometric histograms for stem CD24, CD34, CD49f and CD90 (<b>b</b>), and CD15 (<b>c</b>, arrow identifies the CD15^High population), in control and ethanol-treated (320 mg/dl) cultures. ‘X’ axis indicates fluorescence intensity while ‘Y’ axis indicates cell number. The histogram in the bottom panel in ‘<b>b</b>’ and ‘<b>c</b>’ indicates background immunofluorescence visualized by staining with an isotype-specific antibody, used to gate specific labeling (vertical dashed lines). (<b>d</b>) Quantification of flow cytometric data shows that ethanol significantly decreased the numbers of cells specifically labeled with CD24 or CD15. Asterisks indicate statistically significant differences relative to controls.