EBV-encoded dUTPase released in exosomes induces NF-κB activation through TLR2.

<p>A) Western blot analysis of exosomal marker HSP-70 in extracts from non-induced and induced Raji cells-derived exosomes. B) TLR2-HEK293 expressing cells were transiently transfected with NF-κB reporter gene and pRL-TK transfection control plasmids. After 24–36 h, cells were exposed for 8 h to equal amounts of exosome extracts (13–39 µg) from induced or non-induced Raji cells and luciferase reporter activity measured, as we have described <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0069827#pone.0069827-Ariza1" target="_blank">[12]</a>. Data was normalized for transfection efficiency and reporter activity expressed as the mean relative stimulation ± SD of an n = 4. *<i>p</i><0.05, **<i>p</i><0.01.