LC3 was decreased in HC kidneys and increased in CR kidneys compared with CON kidneys.

<p>LC3 expression was analysed by Western blotting in the renal tissues of the CON, CR and HC Fischer 344 rats. <b>a:</b> Theexpression of LC3-I and LC3-II proteins was detected by Western blot analysis.<b>b:</b> The ratio of the LC3-II to LC3-I bands was analyzed. The protein expression data are presented as the mean ± SD (n = 6). *p<0.05 vs. CON.<b>c:</b> Immunohistochemistry staining results for LC3 proteins and 8-OHdG in the kidneys of the CON, CR and HC Fischer 344 rats were scanned by a microscope.CON, control animals; CR, calorie-restricted diet; HC, high-calorie diet.