Enzymatic properties of CrCaf1.

<p>(A) Determination of the enzymatic parameters by varying the concentration of the substrate A20. The concentration of A20 ranged from 0 to 40 µM, and the concentration of CrCaf1 was 0.05 mg/ml. The reaction time was 10 min. The arrow indicates the direction of increasing the substrate concentration. The inset shows the data fitting using the Michaelis–Menten equation, and the fitted curve is presented as a solid line. (B) Catalytic pattern of CrCaf1 by the SEC method. The SEC profiles were collected at various time intervals. The positions of the substrate A20 and the product AMP are labeled. (C) Characterization of the optimal pH for CrCaf1 reaction. (D) Temperature-dependence of CrCaf1 activity.