MeCP2 interferes with HP1γ binding.

<p>AO3_1 cells (CHO-derived clone containing an integrated lacO array) were co-transfected with EGFP-tagged HP1α, β or γ (green signal) and mCherry-tagged lacR or lacR-MeCP2 (red signal). (A-C) Pictures show 3D images that were recorded of living cells. The images represent individual optical sections and nuclei have the same scale. Bar = 5 μm. (D) The curves show Fluorescent Recovery After Photobleaching (FRAP) data of HP1γ at the lac operator (red curve) as well as at the overall nuclear localization (blue curve) (E) The graphs show FLIP curves of EGFP-HP1γ in the presence of mCherry-lacR-MeCP2 (red line) or mCherry-lacR (blue line). The insets show an EGFP-HP1γ and mCherry-lacR targeted cell of which half of the nucleus is continuously bleached (only green channel is shown). Bar = 5 μm. FLIP was measured in the bottom half of the nucleus.