Tm7sf2 gene controls eIF2α activation and attenuates ATF4 protein levels.

<p>(a) ER stress response in WT and KO MEFs. MEFs, grown in DMEM plus 5%LPDS, were treated with 1 µM thapsigargin for 18 hr, lysed and analyzed by Western blotting with the indicated antibody. β-actin and eIF2α were used as loading controls. (b) Time-course of eIF2α phosphorylation. WT and KO MEFs, treated for the indicated time with 1 µM thapsigargin, were lysed and analyzed for eIF2α phosphorylation. β-actin and eIF2α were used as loading controls. (c) ER stress response activation in mice. Kidneys of WT and KO mice, i.p. injected with 1 mg/Kg tunicamycin and sacrificed at 48 hr, were analyzed by Western blotting with the indicated antibody. β-actin was used as loading control.