NLK induces c-Myb degradation in MCF-7 cells.

<p>(A). MCF-7 cells were transfected with the pcDNA3.1-Myc-NLK or pcDNA3.1-Myc expression vectors, and 32 hours posttransfection, 26S proteasome inhibitor MG132 and DMSO as control were added for 16 hours. At 48 hours posttransfection, whole cell lysates were prepared, and proteins were analyzed by immunoblotting. The data were representative of three experiments. (B). pcDNA3.1-Myc-NLK or empty vector was transfected into MCF-7 cells using Lipofectamine 2000. Western blotting was performed using antibodies against c-myc, Bcl-2 and β-actin. The bar chart demonstrates the ratio of c-myc and Bcl-2 proteins to β-actin as determined by densitometry. The data are represented as the mean ± SEM (n = 3, *, #, P<0.01, compared with control).