Treatment with Ang-1-neutralizing Antibody Suppresses Shh-up-regulated ZO-1 and Occludin in BMECs under OGD.

<p>BMECs were pre-treated with Ang-1-neutralizing antibody (AngAb), and/or PBS, Shh for 30 min, and then subjected to the OGD for 4 h. (A-C) The mRNA levels of ZO-1 (A), occludin (B) and claudin-5 (C) were determined by real-time RT-PCR. Ctrl: cells were pre-treated with PBS and then subjected to normal oxygen condition. AngAb: cells were pre-treated with AngAb and then subjected to normal oxygen condition. OGD: cells were pre-treated with PBS and then subjected to OGD. Shh+OGD: cells were pre-treated with Shh and then subjected to OGD. AngAb+Shh+OGD: cells were pre-treated with Shh plus AngAb and then subjected to OGD. ^Δ<i>P</i><0.01 vs. the Ctrl group, *<i>P</i><0.01 vs. the OGD group, ^#<i>P</i><0.01 vs. the Shh group. Each experiment was repeated three times (n = 6, each group).