The structure of nsPEF-exposed cell populations with and without blockage of cell swelling with sucrose.

<p>Bars show relative fractions of non-apoptotic, apoptotic, and dead cells at different timepoints after nsPEF (600 pulses, 300 ns, 7 kV/cm). Growth media were changed the same way as in Fig. 1. Dead and live cells were counted by the AO/PI assay. The fraction of apoptotic cells among live cells was considered proportional to the fraction of cleaved PARP. The data were averaged from 4–5 experiments; the error bars are omitted for clarity.