Expression of proteins in AmE-711 cells at different temperatures.

<p>The AmE-711 line had been transferred 12 times and incubated for 7 days at different temperatures. Mean total protein per unit surface area of tube substrate (cm²) was determined from 3 replicate cultures per temperature using a Bradford assay. Baseline data represent the amount of protein in cells from 3 replicate cultures harvested within 24 h after inoculation. All cultures were run in triplicate and the data were normalized against a reagent blank (0.5 N NaOH) and BSA standard curve. Mean total protein was significantly different between cultures grown at different temperatures (<i>F</i>_4,10=16.00, <i>p</i>=0.0002). Columns with different letters are significantly different by Tukey’s HSD (α = 0.05).