miR-21 (A) was upregulated and PTEN (B) was downregulated in xenografts, derived from miR-21 over-expressing HCT116 cells (miR-21) and corresponding vector controls (pcmv) in SCID mice as described previously [12].

<p>Quantitative real-time RT PCR was performed with RNA isolated from formalin fixed paraffin embedded xenografts.