Time course of intracellular APP/Aβ immunoreactivity in APP^Swe/PS1^L166P mice.

<p>Representative images of coronal sections of hippocampal pyramidal cell layer (<b>A</b>′), entorhinal (<b>A′′</b>) and primary motor (<b>A′′′</b>) cortex of APP^Swe/PS1^L166P mice, at the peak of intracellular 6E10 immunostaining positivity. Calibration bar, 50 µm. (<b>B</b>-<b>I</b>) Quantitative analysis of APP/Aβ immunoreactivity in the intracellular compartment in hippocampal pyramidal cell layer (<b>B</b>; <b>C</b>), entorhinal (<b>D</b>; <b>E</b>; <b>F</b>) and primary motor cortex (<b>G</b>; <b>H</b>; <b>I</b>) in terms of APP/Aβ+ optical density (<b>B</b>; <b>E</b>; <b>H</b>), hippocampal pyramidal cell layer thickness (<b>C</b>), cell number (<b>D</b>; <b>G</b>) and cell size (<b>F</b>; <b>I</b>) in 1-, 2-, 4-, 6- and 12-month-old APP^Swe/PS1^L166P mice. The APP/Aβ+ optical density of the intracellular compartment in hippocampal pyramidal cell layer (<b>B</b>) and the APP/Aβ+ cell number in both entorhinal (<b>D</b>) and primary motor (<b>G</b>) cortex showed age-related differences. The hippocampal pyramidal cell layer thickness (<b>C</b>) and the APP/Aβ+ cell size in both entorhinal (<b>F</b>) and primary motor cortex (<b>I</b>) showed no age-related differences (n = 5 in each group, three sections per mouse, three frames per section, * <i>P</i><0.05, One-way ANOVA). Error bars represent SEM. (AU, arbitrary units; HIP, hippocampus; EC, entorhinal cortex; PMC, primary motor cortex).