Transcript profiles of <i>ZmSKD1</i> in major organs of maize and its responses to salt and drought stresses.

<p><b>A</b> The transcriptional pattern of <i>ZmSKD1</i> in maize root (R), stem (St), leaf (L), tassel (Ta) and immature ear (Ie) samples evaluated by real-time PCR. For each real-time PCR, the transcript levels of maize internal control gene <i>Ubiquitin</i> were also evaluated in various samples. For each assay, the expression level in stems was defined as 1.0, and data represented means ± SE of three technical replicates. *t-test, with P<0.05. <b>B</b> Time-course analysis of <i>ZmSKD1</i> transcript levels under various concentrations of salt treatments by real-time PCR. Two-week-old maize seedlings were exposed to 100, 200, and 300 mM NaCl for indicated time points (0, 6, 12, 24, and 48 h), and leaf samples were used for real-time PCR analysis. <b>C</b> Time-course analysis of <i>ZmSKD1</i> transcript levels under PEG-induced water stress by real-time PCR. Two-week-old maize seedlings were exposed to 0, 10%, 15%, and 20% PEG6000 for indicated time points (0, 6, 12, 24, and 48 h), and leaf samples were used for real-time PCR analysis. In both B and C assays, <i>Ubiquitin</i> was used as an internal control. For each treatment, the expression level at time point 0 was defined as 1.0, and data represented means ± SE of three technical replicates. **t-test, with P<0.01; *t-test, with P<0.05.