Changes to the phospho-proteome upon CK1 modulation.

<p>(<b>A</b>) Volcano plot visualising quantified phospho-sites with significant differences between CK1-OX8 and CCA1-LUC in a pair-wise comparison, using label-free quantification by mass spectrometry. Criteria were p<0.05 and fold changes >1.5 or <0.67, as indicated by black lines. Blue data-points represent significantly differential phospho-sites, black circles represent those that do not change significantly. The identities of these sites can be found in Supplementary Dataset S1. (<b>B</b>–<b>C</b>) Volcano plots visualising quantified phospho-sites with significant differences between mock-treated cells and cells inhibited for CK1 using IC-261 (B) or PF-670462 (C) in pair-wise comparisons, using label-free quantification by mass spectrometry. Criteria were p<0.05 and fold changes >1.5 or <0.67, as indicated by black lines. Red data-points represent significantly differential phospho-sites, black circles represent those that do not change significantly. The identities of these sites can be found in Supplementary Dataset S2 (D) Chord diagram showing the numbers of identified phospho-sites whose abundance significantly differs upon drug treatment with either IC261 or PF-670462 from their respective controls (>1.5-fold change, p<0.05, n=5), and the overlap between the indicated data sets. Up-regulated peptides are represented in dark red, down-regulated peptides in lighter red.