OpgH^N is an inhibitor of FtsZ assembly.

<p>(A) Induction of OpgH inhibits FtsZ assembly in vivo. Cells were sampled and imaged for immunofluorescence microscopy at 25 minute intervals following the induction of either <i>thio-his</i> (black squares), <i>thio</i>-<i>opgH</i>-<i>his</i> (dark grey diamonds), or <i>thio</i>-<i>opgH^N</i>-<i>his</i> (light grey circles). Cells were cultured in LB. >200 cells were evaluated per sample. Representative α-FtsZ immunofluorescence micrographs from time points 0′ and 150′ are shown in the lower left. (B) Mutations that disrupt synthesis of UDP-glucose or OpgH itself suppress the lethality of MinD overexpression. MinD is overexpressed by ∼2-fold, which is at the threshold of lethality in WT. Error bars equals standard deviation (n = 3). (See <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003663#pgen.1003663.s002" target="_blank">Figure S2</a> for relative MinD expression levels.) (C) A representative 90° angle light scattering plot of FtsZ assembly ± OpgH^N. FtsZ is at 5 µM, OpgH^N is at 10 µM. Arrow indicates addition of 1 mM GTP. (D) Concentration-dependent inhibition of FtsZ polymerization by OpgH^N. The ratio of FtsZ to OpgH^N is listed below. FtsZ is at 5 µM in all cases. (See <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003663#pgen.1003663.s009" target="_blank">Figure S9A, S9B</a> for additional controls.)