Inferred network edge validation.

<p>A) Network edges were compared to a predicted transcription factor – target database. The number of transcriptome network edges for each virus that was also present in the database (red) was compared with the number of matching edges in 1000 random networks (gray) to estimate the number of matching edges expected from chance. B) Relationships between genes targeted in a large siRNA-targeting study <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0069374#pone.0069374-Hurley1" target="_blank">[19]</a> and the downstream affected genes were compared to relationships predicted from our network inference approach. Results show the number of genes that exhibited statistically significant overlap between their network neighbors and perturbed genes from the siRNA targeting study. Red designates the overlap with neighbors from the actual network; grey designates overlap with neighbors from 500 random networks (see Materials and Methods). Error bars represent standard deviation of the distribution of gene percentages with significant overlaps.