Effect of BP on phosphorylation of Akt and GSK3β in 3T3-L1 adipocytes.

<p>(A) Effect of BP on Akt activation in 3T3-L1 adipocytes. 3T3-L1 adipocytes were treated with BP extracts at the indicated concentrations and the phosphorylation levels for Akt was determined by Western blotting analysis. The data are presented as the means ± SD values for at least three independent experiments. *P<0.05. (B) Effect of BP on GSK3β activation in 3T3-L1 adipocytes. 3T3-L1 adipocytes were treated with BP extracts at the indicated concentrations, and the phosphorylation levels for GSK3β were determined by Western blotting analysis. The data are presented as the means ± SD values of at least three independent experiments. *P<0.05. (C) Effects of the PI3K/Akt inhibitor LY294002 (10 µM) on BP-induced inhibition of adipocyte differentiation in 3T3-L1 cells. 3T3-L1 cells were treated with BPE during differentiation in the presence or absence of the LY294002. The intracellular lipid accumulation was measured by triglyceride assay. Data are expressed as mean ± SD of three independent experiments. *P<0.05.