Tumor radiation results in a myeloid contraction.

<p>a) i) Flow cytometry of fresh whole peripheral blood from naïve and 4T1 tumor-bearing BALB/c mice, showing CD11b⁺SSC^hi myeloid populations. ii) Gr1 and IA (MHC class II) staining on gated CD11b⁺SSC^hi myeloid populations from naïve and 4T1 tumor-bearing mice. b) i) Mean and standard error of leg diameter of BALB/c mice bearing 4T1 tumors left untreated (NT) or treated beginning on day 14 with 3 daily doses of 20 Gy focal radiation (RT). Myeloid cells/µl peripheral blood of ii) mice left untreated and iii) mice treated beginning on day 14 with 3 daily doses of 20 Gy focal radiation. iv) fitted curves from graphs ii) and iii) plotted without measurements. c) i) Clonogenic assays of lung metastases present at euthanasia in BALB/c mice bearing 4T1 tumors left untreated (empty circles – NT) or treated beginning on day 14 with 3 daily doses of 20 Gy focal radiation (filled circles – RT). ii) Myeloid cells/µl peripheral blood of naïve mice (Naïve) and mice day 17 following injection of 4T1 i.v. (i.v.) or s.c. (s.c.) iii) Total spleen cellularity in naïve mice (NT) and mice day 24 following injection of 4T1 i.v. (i.v.) or s.c. (s.c.) d) i) Myeloid cells/µl peripheral blood of C57BL/6 mice bearing Panc02 tumor harvested at different time points. ii) day 24 leg diameter and iii) myeloid cells/µl peripheral blood of C57BL/6 mice bearing Panc02 tumor left untreated (NT) or treated beginning on day 14 with 3 daily doses of 20 Gy focal radiation (RT). In each graph, each symbol represents one mouse. NS = Not significant; * = p<0.05; ** = p<0.01; *** = p<0.005; **** = p<0.001. Data represents multiple replicate experiments.