Verification of the E2 peptide epitopes recognized by PAbs raised in different avian.

<p>E2-reactive PAbs were elicited in chickens and ducks immunized with purified recombinant EEEV E2 protein. A Western blot screen of forty-two 16-mer peptides derived from the E2 protein sequence identified a panel of putative peptide epitopes recognized by the E2-reactive PAbs of one or more species. The candidate epitopes were synthesized as short peptides and used in an ELISA to measure PAb binding to each linear peptide epitope. PAb binding was determined by measuring optical density (OD) at 492 nm. Error bars indicate standard deviation. Positive value/Negative value ≥2.1 was considered as positive result.