Blocking JAM-A and ALCAM Inhibits CCL2-Mediated Increase in HIV Infected Monocyte Transmigration Across the BBB.

<p>HIV infected or uninfected monocytes from 4 independent individuals were added to the BBB model and transmigrated in response to CCL2 in the presence of blocking antibodies to (<b>A</b>) JAM-A or (<b>B</b>) ALCAM. The number of CD14⁺CD16⁺ monocytes that transmigrated was analyzed by flow cytometry. CCL2 (horizontal hatching) promoted increased transmigration compared to media alone (white bars) for both uninfected and HIV infected cells. (<b>A</b>–<b>B</b>) HIV infected monocytes transmigrated in significantly greater numbers relative to uninfected monocytes in response to CCL2. Blocking antibodies (vertical hatching) to (<b>A</b>) JAM-A and (<b>B</b>) ALCAM decreased monocyte transmigration similarly for both HIV infected and uninfected monocytes. An isotype matched negative control antibody (diagonal hatching) did not affect CCL2-mediated transmigration. Data are represented as mean ± standard error of the mean. Significance was determined using a Two-Tailed Paired T test. ^*p<0.05**p<0.01.