HIV Infected Monocytes Have Highly Increased Sensitivity to CCL2.

<p>Chemotaxis across a 5 µm polycarbonate membrane to increasing concentrations of CCL2 ranging from 1–500 ng/mL was examined in uninfected and HIV infected monocytes derived from eight independent donors. HIV infection (black bars) significantly increased monocyte chemotaxis to CCL2 relative to uninfected cells (white bars) at all concentrations. Uninfected cells did not chemotax to concentrations below 25 ng/mL, whereas HIV infected cells migrated even to very low levels of CCL2 (1 and 10 ng/mL). Data are represented as mean ± standard error of the mean. Significance was determined using a Wilcoxon Signed Rank test. ^#p<0.05 indicates significant chemotaxis relative to 0 ng/mL CCL2 for the uninfected cells. ^&p<0.01 indicates significant chemotaxis relative to 0 ng/mL CCL2 for the HIV infected cells. ^*p<0.05 and **p<0.01 indicate significant chemotaxis of HIV infected cells relative to uninfected cells. NS indicates no significant change as compared to 0 ng/mL CCL2 for the uninfected cells.