JAM-A and ALCAM Increase on CD14⁺CD16⁺ Monocytes Upon HIV Infection.

<p>The surface expression of JAM-A (<b>A</b>) and ALCAM (<b>B</b>) was analyzed by flow cytometry on HIV infected or uninfected monocytes from 20 independent individuals. After subtracting the contribution of the isotype matched negative control antibodies, the fold increase in the MFI of each junctional protein on the monocytes that were cultured nonadherently and were either HIV infected (diamonds) or remained uninfected (triangles), relative to the freshly isolated monocytes (circles, set to 1) was determined. The majority of the individuals (11/20) had an additional increase in JAM-A and ALCAM after HIV infection over that which had already occurred during monocyte maturation (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0069270#pone-0069270-g005" target="_blank">Figure 5)</a>. Monocytes from the same 11 individuals had an increase in both JAM-A and ALCAM. Data are represented as mean ± standard error of the mean. Significance was determined using Wilcoxon Signed Rank test. **p<0.01.