The protein levels of mouse Tdp-43 are reduced in human TDP-43 transgenic mice.

<p>(<b>A</b>) Generation and characterization of an antibody that specifically detects mouse Tdp-43 (mTdp-43 antibody). Schematic representation of mouse TDP-43 protein and the amino acid residues used as immunizing peptide. Western blot analyses of lysates from mouse brain of non-transgenic (NT) and human TDP-43 transgenic (Tg) mouse, and human M17 neuroblastoma cells. Before proceeding with the staining protocol, mTdp-43 antibody is neutralized with immunizing peptide. The neutralized antibody is then used side-by-side as a control for the antibody alone. Our results showed that our mTdp-43 antibody can specifically recognize mTdp-43 in both NT and hTDP-43 Tg mice brain, but cannot recognize human TDP-43 in human sample. Human TDP-43 antibody (hTDP-43) was used to confirm the expression of human TDP-43 in samples. (<b>B</b>) Compared to NT mice, significant reduction of mTdp-43 protein was observed in both TDP-43 hemizygous and homozygous mice brain. Data shown are the means ± SEM of 5 mice per group; *p<0.05, **p<0.001, as assessed by one-way ANOVA with Tukey’s posthoc analysis.