Suppressing GLUT4 with GLUT4 siRNA reduced the cardioprotection of IPC.

<p>(A) GLUT4 mRNA expression (n = 4/group); (B) GLUT4 protein expression, Top images: representative blots (n = 4/group); (C) myocardial infarct size expressed as percentage of area at risk (AAR, n = 6/group). All rats were subjected to IPC and 30 min of ischemia followed by 1 h of reperfusion for qPCR and western analysis or 3h of reperfusion for the determination of myocardial injury. IPC was induced by 2 cycles of 5 min of ischemia/5 min of reperfusion. NC, negative control. Values presented are means ± SEM.<i>**P</i><0.01 vs. NC SiRNA.