Immunohistochemical staining for 4-HNE in cultured rat hepatic stellate cells (×200).

<p>(A) Mild staining for 4-HNE was present in untreated control cells and cells treated with BSA. Marked staining for 4-HNE was present in stellate cells treated with both 5 µg/ml and 10 µg/ml AA-AGE. A few apoptotic cells (arrow) observed in cultures treated with both 5 µg/ml and 10 µg/ml AA-AGE. (B) Quantitative representation of the staining intensity of 4-HNE in cultured stellate cells (Mean ± S.D., <i>n = 5</i>).