Multiphoton laser tomography and fluorescence lifetime imaging of melanoma.

<p>A, MPT image of upper melanoma layers; B, FLIM image of the same spot displayed by a 0–2000 <i>ps</i> fluorescence lifetime range showing that all cells are melanocytic; C, 0–400 scale, employed to increase contrast for the distinction of cellular details, such as the cytoplasmic halo and the speckling. Dots indicate nuclei with undefined contours; arrows indicate polinucleated cells; asterisks indicate cytoplasmic halo, and triangles speckled melanin. FLIM values do not change, they are only displayed in a different way.