Ficus arrests the cell cycle in SiHa at G₁/S phase and modulates the expression of cell cycle regulatory proteins.

<p>SiHa cells were treated with different concentrations of FR_aq (0–80 µg/ml) for 24 h. (<b>A</b>) Enhanced accumulation of the cells in G₁ phase with a concomitant decrease in S-phase population was observed after treatment with Ficus (as indicated by histograms). Western blot shows the expression levels of p53 and pRb (<b>B</b>) as well as p21 and ppRb (<b>C</b>). Tubulin was used as a loading control. (<b>D, E</b>) Densitometric analysis of the western blot showing fold change in protein levels upon FR_aqtreatment. The bands were quantified by densitometry scanning using ImageJ 1.44p (Wayne Rasband, National Institutes of Health, USA, <a href="http://imagej.nih.gov/ij" target="_blank">http://imagej.nih.gov/ij</a>). The data represents mean ± SD of three independent experiments.