Ficus regulates invasion and migration of cervical cancer cells.

<p>Analysis of cell migration in SiHa (<b>A</b>) and HeLa (<b>B</b>) treated with FR_aq (0–80 µg/ml) was measured by wound-healing assay. The upper panel of the image shows the wound made at 0 h. The lower panel shows the migration of cells corresponding to the distance travelled at 16 h. (<b>C</b>) Graphical representation of wound closure in SiHa and HeLa cells at 16 h after FR_aq treatment has been shown. Values were represented as the percent wound closure and expressed as mean ± SD for three independent experiments. (<b>D</b>) Cell invasion assay showing the percentage of cells invaded per field in the presence or absence of FR<b>_aq</b>. The invaded cells were counted in ten random fields and the values have been expressed as mean ± SD for three independent experiments. (<b>E</b>) Gelatin zymography showing downregulation of MMP-2 expression in FR<b>_aq</b> (0–80 µg/ml) treated SiHa and HeLa. (<b>F</b>) Western blot analysis showing decrease in Her-2 expression in SiHa and HeLa treated with FR<b>_aq</b> (0–80 µg/ml). Tubulin was used as a loading control. (<b>G</b>) Densitometric analysis of the western blot showing fold change in HER-2 protein levels in SiHa and HeLa. The bands were quantified by densitometry using ImageJ 1.44p (Wayne Rasband, National Institutes of Health, USA, <a href="http://imagej.nih.gov/ij" target="_blank">http://imagej.nih.gov/ij</a>).