Immunohistochemical analyses of original colorectal cancers and their metastatic lymph nodes.

<p>Thirty-three cases of original colorectal cancer tissues and four cases of their metastatic lymph nodes were stained with anti-GMDS antibody and AAL. (A) Cases 4 and 6, which depict the original colorectal cancer with GMDS mutation, but not case N, which did not have GMDS mutation, showed negative staining for both GMDS and AAL. (B, C) In case L-6, which did harbor GMDS mutation, the metastatic lymph node was not stained for GMDS (B) and AAL (C) despite positive staining for both GMDS and AAL in the original colorectal cancer tissue sample. Case L-4 without GMDS mutation showed positive staining for GMDS and AAL in both the original and metastatic legions. Bar indicates 100 µm. LN, lymph node.