Conditioned media from SW480^FJX1 cells enhances endothelial capillary tube formation <i>in vitro</i>.

<p>(<b>A</b>) Relative number of HMEC-1 tube structures following treatment with FJX1 conditioned media as compared with VEC conditioned media in the presence (10%) and absence (0%) of serum. (<b>B</b>) Representative immunoblot of SW480^FJX1MYC cells treated with siRNA specific to <i>FJX1</i> (siFJX1) or scrambled control oligonucleotide (siSCR) probed with anti-FJX1. β-actin and Coomassie stain served as loading controls for whole cell lysate (WCL) and conditioned media (CM), respectively. Asterisks (*) indicate non-specific band. (<b>C</b>) Relative number of HMEC-1 tube structures formed in the presence of conditioned media from SW480 cells treated as noted as compared to VEC untreated with siRNA (UT). (<b>D</b>) Relative number of HMEC-1 tube structures as compared with VEC after fractionation of the conditioned media. RET = retained on column; FT = flow through. For all graphs, each data point represents the value of a biological replicate and bars and whiskers represent the mean and standard error of the replicates, respectively. Significance was determined by Student's t-test; *P<0.05; **P<0.005; ns = not significant. (VEC = SW480^VEC and FJX1 = SW480^FJX1MYC conditioned media. UT = untreated. siSCR = treated with scrambled siRNA. siFJX1 = treated with FJX1 siRNA.