ROS promoted aggressive cellular behaviors by up-regulating HIF-1α expression.

<p>A, The sequence of inserts in the shRNA vector clone. B, Cells with HIF-1α shRNA showed decreased HIF-1α expression compared with the control. C, Cells with HIF-1α shRNA showed decreased migration ability compared with the control as documented by transwell migration assay. **P<0.05 vs A clone (n = 3) D, Cells with HIF-1α shRNA showed decreased invasive capacity compared with the control as documented by transwell invasion assay. **P<0.05 vs A clone (n = 3) E, Wound healing assay showed that cells with HIF-1α shRNA migrated slower than the control.