Ion content and expression analysis of transporter genes in transgenic lines and controls (VC and WT) under normal and salt stress conditions.

<p>Two-week-old seedlings were transplanted to MS or MS with 200 mM NaCl for one week. Whole seedlings were sampled to measure the content of Na⁺, K⁺, Ca²⁺ and the ratio of K⁺/Na⁺ was calculated (A). Two-week-old tobacco seedlings were transplanted to MS or MS with 200 mM NaCl for two days and the whole seedlings were used to measure the expression of transporter genes (B). Data are means ±SD calculated from four replicates. Asterisks indicate significant difference between the WT and the three transgenic lines (*<i>p</i><0.05; **<i>p</i><0.01). Three different experiments were performed with similar results.