Genome-wide scans for the percentage of body fat (% body fat) of C57BL/6ByJ x 129P3/J F₂ male and female mice fed low- and then high-energy diet (Experiment 1).

<p>Horizontal lines represent suggestive linkage thresholds determined by 1000 permutation tests [threshold = 3.08 when mice were fed the low-energy diet (dashed lines) and 3.77 when fed the high-energy diet (solid lines)]. (A and B) Sex-dependent QTLs are determined by the difference between the LOD scores when sex is used as an additive versus a combined additive and interactive covariate. Significant differences between the additive (dashed line) and additive plus interactive (solid line) models after the low-energy (A) and high-energy (B) diets are indicated by arrows. (C) Diet-dependent effects were determined by the difference in LOD scores between each diet period (arrows). The y-axis lists chromosome numbers. All analyses included age and litter size as covariates, and the diet-specific analysis in C was conducted using sex as an interactive covariate. Details about individual QTLs are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068776#pone-0068776-t002" target="_blank"><b>Table 2</b></a>.