HSP47 expression affected mitochondria caspase-9 cleavage.

<p>(A) NIH3T3 cells treated with GalNAc-bn for the indicated periods were examined by western blot analysis using an antibody against caspase-9. NIH3T3 cells treated with staurosporine (STS) were used as a positive control for caspase-9 cleavage induction. Cont, untransfected cells; Scr, scrambled siRNA-transfected cells; siRNA, HSP47 siRNA-transfected cells; NoS, no stimulated cells. (B) CAG and BCL cells treated with STS for 12 h were examined by western blot analysis using an antibody against caspase-9. (C) Western blot analysis showed caspase-2 and GAPDH protein expression 2 d after transfection with caspase-2 siRNA. (D) CAG, BCL, and caspase-2 siRNA transfected NIH3T3 (Cas2 siRNA) cells treated with GalNAc-bn for 24 h were examined by western blot analysis using an antibody against caspase-9 after HSP47 siRNA transfection.