Golgi stress-induced elevation of HSP47 protein expression.

<p>(A, B) Western blotting analysis showed a remarkable and dose-dependent increase in HSP47 protein levels after GalNAc-bn stimulation (A, Colo 205 cells; B, NIH3T3 cells). (C) Quantification of the protein bands in NIH3T3 cell samples after 1-d treatment with GalNAc-bn, Tm, or Tg. Data are expressed as mean (SEM) of at least 3 independent experiments. ***p < 0.01 (Student’s <i>t</i> test). Tm, tunicamycin; Tg, thapsigargin. (D) Western blotting analysis showed a dose-dependent increase in HSP47 protein levels after monensin stimulation for 12 h in NIH3T3 cells.