Influence of the crowder type on IAPP restriction and binding.

<p><b>A</b> Crowder concentration-dependent lateral diffusion constant, <i>D</i>, of 10 µM fluorescent labeled rIAPP (rIAPP-K-Bodipy FL) as monitored by FCS. The polymer-like network forming molecules Ficoll and dextran as well as the proteins BSA and lysozyme were used as crowding reagents at concentrations ranging from 10 µM to 40% (w/v). <b>B</b> Content of rIAPP bound to the different crowding agents as analyzed from the FCS results of the equimolar rIAPP-K-Bodipy FL to crowder mixture (10 µM each). Data points are mean values (errors bars indicate standard deviations) of ≥3 individual measurements.