M1 polarization is inhibited in BMMΦ from obese mice after <i>P.</i><i>gingivalis</i> infection.

<p>(A) Uninfected BMMΦ from lean (LN) and obese (OB) mice were left unlabeled or were labeled with PE-conjugated anti-mouse CD11b and analyzed by FACS to determine macrophages differentiation. (B) Uninfected BMMΦ from LN and OB mice were left unlabeled or were labeled with PE-conjugated anti-mouse F4/80 and analyzed by FACS to determine macrophages maturation. (C) BMMΦ from LN and OB mice were infected with <i>P. gingivalis</i> (P.g) for 1, 4 and 24 h. Cells were collected and labeled with PE-conjugated anti-mouse IL-12 and FITC-conjugated anti-mouse IL-10. The percentages of M1 and M2 macrophages were measured by FACS analysis. Data represent one of three independent experiments with consistent results. (D) Percentage of BMMΦ from LN and OB mice that present an M1 polarization (high levels of IL-12 and low levels of IL-10) and an M2 polarization (high levels of IL-10 and low levels of IL-12).