Non-adherent sphere formation assays of EpCAM<sup>+</sup> cells treated with metformin.
- Tomoko Saito (585310)
- Tetsuhiro Chiba (508754)
- Kaori Yuki (508756)
- Yoh Zen (307071)
- Motohiko Oshima (165144)
- Shuhei Koide (508757)
- Tenyu Motoyama (508758)
- Sadahisa Ogasawara (508759)
- Eiichiro Suzuki (508755)
- Yoshihiko Ooka (508760)
- Akinobu Tawada (508761)
- Motohisa Tada (585311)
- Fumihiko Kanai (59638)
- Yuichi Takiguchi (395734)
- Atsushi Iwama (46665)
- Osamu Yokosuka (234198)
Publication date
July 2013
DOIAbstract
<p>(A) Western blot analysis of EpCAM expression in sorted EpCAM<sup>+</sup> cells. Tubulin was used as a loading control. (B) Bright-field images of the non-adherent spheres of EpCAM<sup>+</sup> HCC cells at day 14 of culture. Scale bar = 100 µm. (C) Number of large spheres generated from 1,000 EpCAM<sup>+</sup> cells treated with metformin. *Statistically significant (p<0.05). (D) Number of secondary spheres 14 days after replating. *Statistically significant (p<0.05).</p
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